Journal: International Journal of Medical Sciences

Article Title: Identification of an Anoikis-associated LncRNA Signature to Predict the Clinical Prognosis and Immune Function of Patients with Endometrial Cancer

doi: 10.7150/ijms.107243

Figure Lengend Snippet: (A) KM Plotter database exhibited the relationship between expression of CFAP58-DT and OS in EC patients. (B) qRT-PCR showed the CFAP59-DT expression in different EC cells. (C-H) CCK8, EdU, Calcein/PI cell staining, flow cytometry and colony formation assays showed shCFAP59-DT enhanced the apoptosis and inhibited proliferation of KLE cells. (I) Western blot showed the protein levels of BAX, Bcl2, Caspase3, Active Caspase3.β-Actin is an internal parameter.

Article Snippet: After incubation for 24 h, the medium in each well was replaced with fresh medium containing 10% CCK8 (ABclonal, China) and incubated for 1-2 h in the dark at a constant temperature.

Techniques: Expressing, Quantitative RT-PCR, Staining, Flow Cytometry, Western Blot

Journal: Molecular Medicine

Article Title: Liraglutide attenuates type 2 diabetes mellitus-associated non-alcoholic fatty liver disease by activating AMPK/ACC signaling and inhibiting ferroptosis

doi: 10.1186/s10020-023-00721-7

Figure Lengend Snippet: Liraglutide inhibits sensitivity of high glucose on ferroptosis. A Cell viability in HepG2 cells treated with indicated concentrations of high glucose (HG) for 24 h and 48 h. B Cell viability in Hep3B cells treated with HG (125 mM, 48 h) and combined with 5 µM Z-VAD-FMK (Z-VAD, 24 h), 2 µM Necrostatin-1 s (Nec-1 s, 24 h) and 5 µM ferrostatin-1 (Fer-1, 24 h) . Bar graphs showing cell viability in indicated cells treated with HG (125 mM, 48 h) or erastin (10 µM, 24 h) (C) , RSL3 (1.0 µM, 24 h) D and combined with 5 µM Z-VAD-FMK (Z-VAD, 24 h), 2 µM Necrostatin-1 s (Nec-1 s, 24 h) and 5 µM ferrostatin-1 (Fer-1, 24 h). PLC cells viability were measured with 1 mM, 5 mM, 100 mM glucose (48 h) combined with erastin (24 h) E or RSL3 (24 h) (F) . G Cell viability measured in HepG2 cells treated with 125 mM HG and different concentrations of liraglutide for 48 h. H – I HepG2 and Hep3B cells were treated with 100 mM HG, erastin (5 μM) and combined with 1.0 μM liraglutide for 24 h. J , K HepG2 and Hep3B cells were treated with 100 mM HG, RSL3 (0.5 μM) and combined with 1.0 μM liragluide for 24 h. Cell viability was determined by CCK-8 assays. L Cell viability measured in HepG2 cells treated with different concentrations of erastin (24 h) combined with 2.0 μM liraglutide (48 h). M Cell viability measured in HepG2 cells treated with different concentrations of RSL3 (24 h) combined with 2.0 μM liraglutide (48 h). N Representative images from immunohistochemical staining of liver tissue microarray for Cleaved caspase-3 and 4-HNE (scale bar, 50 μm). O Representative Fe 2+ fluorescence images of HepG2 cell (scale bar, 100 μm) Data are expressed as mean ± SD, n = 4

Article Snippet: Cell viability was measured as described previously using the Cell Counting Kit-8 (CCK8) (Enogene, Nanjing, China).

Techniques: CCK-8 Assay, Immunohistochemistry, Staining, Microarray, Fluorescence